Antibacterial Activity of Silver Nanoparticles Prepared from Camellia Sinensis Extracts in Multi-Drug Resistant Pseudomonas aeruginosa
Rami Altameemi, Ahmed Harbi AL-Azawi, Hawazen Haleem Salih
The purpose of this study to synthesize and characterize silver nanoparticles using phenolic compounds obtained from Camellia sinensis, to test the antibacterial properties of biosynthesized nanoparticles on the formation of biofilms in multidrug-resistant Pseudomonas aeruginosa. Ten isolates of P. aeruginosa were obtained from the Genetic Engineering and Biotechnology Institute laboratories of the University of Baghdad. By using the VITEK-2 system and culturing the isolates on cetrimide agar, the diagnosis was confirmed. Camellia sinensis silver nanoparticles (CAgNPs) were created using an extract of the plant's aqueous and methanolic leaves. Based on the results of the nanoparticle synthesis, spherical nanoparticles that may be single or mixed were included in both the aqueous and methanolic extracts of silver nanoparticles. By comparing their retention times to those of the reference compounds, the HPLC findings revealed that two phenolic compounds (gallic acid and caffeine) had been discovered. Utilising the disc diffusion technique, the antibacterial activity of (CAgNPs) was assessed. The results indicated that the methanolic (CAgNPs) extract was more effective than the aqueous (CAgNPs) extract at 375 and 750 ppm, giving the highest inhibition zone 17.67 and 21.33 mm, respectively, when compared to the aqueous (CAgNPs) extract, which produced inhibitory zones 13.00 and 16.33 mm, respectively. The MIC findings indicated that the methanolic CAgNPs extract was more effective than the aqueous CAgNPs extract; the MIC of the methanolic CAgNPs extract was 23.43 µg/ml in all P. aeruginosa isolates, except the isolates No. 9 and 10, which was 11.718 µg/ml. While in the aqueous extract, the MIC in all P. aeruginosa isolates was 187.5 µg/ml, with the exception of isolates No. 9 and 10, which was 93.75 µg/ml. Additionally, the methanolic CAgNPs extract entirely inhibited P. aeruginosa from building a biofilm when used at 23.43 µg/ml. However, at 46.87 µg/ml of the aqueous CAgNPs extract, totally reduced the biofilm forming activity on P. aeruginosa isolates.
Keywords: P. aeruginosa, C. sinensis, Nanoparticles, Antibacterial, Antibiofilm
