Whole Genome Sequence Based Analysis of Heavy Metals Genes and Antibiotics Resistance Genes in P. Aeruginosa Isolated from Hospitals in Iraq
Omar A.Al-Hashimi, Safaa Abed Lateef Al Meani,
Ibrahim Omar Saeed
Genes of resistance to heavy metals (HMRS) were detected in five Pseudomonas aeruginosa strains obtained from the hospitals under study, including environmental samples and clinical samples. Resistance isolates were assessed depending on their place of origin and possible exposure to contaminated heavy metals. The antibiotic resistance of these five isolates was examined using the Vitek-2 technology, as well as disinfectant resistance by spreading on dens, which revealed significant antibiotic resistance and insensitivity to disinfectants often used in hospitals. Metal resistance-encoding genes were identified by WGS using NGS technology. Metals resistance genes and antibiotic resistance genes were detected in all five isolates. The presence of resistance-encoding genes in contemporary isolates suggests a link to acquiring resistance features from external sources..
Keywords: P. aeruginosa, WGS, NGS, Heavy Metals, Resistance Genes, Hospital environments
