Molecular Identification of Some Multidrug Resistance Gram Negatives Bacteria from Clinical Mastitis Cow and Study the Antimicrobial Activity of Thymus Vulgaris in Basrah Provence
Ali B. Aldeewan, Alyaa S. Jasim, Basil A. Abbas, Nawres N. Jaber
Bovine mastitis, udder parenchyma inflammation, causes glandular tissue pathology and milk abnormalities. These developments affect the global dairy industry economically. Due to the growing importance of Gram-negative bacteria like Escherichia coli and P. aeruginosa, clinical mastitis (CM) incidences are projected to rise. form 20 isolates that suspected as Pseudomonas aeruginosa and Escherichia coli showed that eight isolates were diagnosed as Pseudomonas aeruginosa and five isolates were diagnosed as Escherichia coli. The antimicrobial susceptibility test against eight strains of Pseudomonas aeruginosa showed that (85%) isolates were resistant to Tetracycline and Gentamicin and (75%) isolates for each of Chloramphenicol and Novobiocin while the antimicrobial susceptibility test against five strains of Escherichia coli Showed that ( 100% ) isolates were resistance for Chloramphenicol, ( 80% ) and ( 60%) isolates were resistance to Ciprofloxacin, Tetracycline and Gentamicin respectively. The current study sought to determine the antimicrobial activity of Thymus vulgaris against resistant Pseudomonas aeruginosa and Escherichia coli. The hot alcoholic extract and the hot water extract were found to be more effective against Escherichia coli than Pseudomonas aeruginosa, and the heated alcoholic extract was more effective than the cool alcoholic extract and the hot water extract.
Keywords: Thymus vulgaris, Escherichia coli, Pseudomonas aeruginosa
