Assessment of Seminal Plasma and Serum Chitotriosidase-1 as Biomarkers for Silent Inflammation in Infertile Iraqi Males: A Comparative Study among Normozoospermic, Asthenospermia, and Oligozoospermic Groups
Ali Abdul Aziz, Hedef El-Yaseen, Hussain Kadhem
The aim of this study was to evaluate the concentrations and activities of cHit1 in the seminal plasma and serum of Normozoospermic, Asthenospermia, and Oligozoospermic groups in relation of serum IL-1b. 90 males aged between 20-45 were included in a cross-sectional study conducted at the infertility unit of Al-Batool Teaching Hospital from February 2022 to February 2023. All participants were diagnosed with infertility by specialists at the hospital. The subjects were categorized into three groups: the Normozoospermic Group (G1), the Asthenospermia Group (G2), and the Oligozoospermic Group (G3). Measurements Interleukin1Beta (IL1B), and Chitinase1 (CHIT1) by Enzyme-linked immunosorbent assay (ELISA) technique. Significantly higher levels of serum IL1B, Serum Ch1t1 and SF Ch1t1 and were found in G2 group compared to G3 and G1 groups. In the G2 group, a moderate positive correlation was observed between Serum Ch1t1 and SF Ch1t1, which was statistically significant. However, a weak positive correlation between Serum Ch1t1 and IL1B was found to be not statistically significant. The correlation between SF Ch1t1 and IL1B was deemed negligible due to the lack of a significant correlation. In the Oligozoospermic group, a weakly negative correlation was noted between Serum Ch1t1 and SF Ch1t1, which was not statistically significant. Regarding IL1B, Serum Ch1t1 showed a weak, non-significant positive correlation, while SF Ch1t1exhibited a strong, statistically significant negative correlation with IL1B. Finally, in the correlation analysis among Serum Ch1t1 and SF Ch1t1, in the three study groups (G1, G2, and G3), a moderate negative correlation was observed but was statistically insignificant in G1. In G2, a significant positive correlation was found between Serum Serum Ch1t1 and SF Ch1t1. The average negative correlation demonstrated between iL-1β concentrations and cHit1 concentrations seem to confirm the hypothesis about the role of cHit1 in the manifestation of inflammation at a very early stage.
Keywords: Human Seminal Plasma chitotriosidase-1, Human Serum Chitotriosidase-1 Inflammation, Serum IL-1B
