Antibacterial and Antibiofilm Activity of Phenolic Compounds Extracted From Camellia Sinensis And Evaluate The Effect On The Gene Expression (Clfa) In Staphylococcus Aureus
Zeena F. Al-Aboudi, Ahmed H. AL-Azawi
The purpose of this study to extract phenolic compounds from Camellia sinensis, investigate their antibacterial and anti-biofilm properties and apply these chemicals to certain virulence genes that are involved in the production of biofilms in Staphylococcus aureus isolates. From laboratories of the Institute of Genetic Eng. and Biotech. at Baghdad university, twenty five isolates of S. aureus were obtained. VITEK-2 technology and growing isolates on Mannitol salt agar verified the diagnosis. Both methanolic and aqueous extracts of Camellia sinensis leaves were made using a Soxhlet equipment and maceration method, In addition to detecting the phenolic compound using high performance liquid chromatography (HPLC) technology. The results indicated that, at concentrations of 128 and 256 mg/ml, For every S. aureus isolate, the methanolic extract outperformed the aqueous extract in effectiveness. giving the highest inhibition zone of 20.33 and 24.67 mm, respectively, while the aqueous extract gave inhibition zones of 11.33 and 13.33 mm, respectively. All S. aureus isolates had a minimum inhibitory concentration (MIC) of 4 mg/ml for the methanolic extract, except isolates No. 7 and 8, which had MIC of 2 mg/ml, and isolate No.10, which had MIC of 8 mg/ml. However, all S. aureus isolates had MIC values of 16 mg/ml for aqueous extract. At 4 mg/ml, the methanolic leaf extract of C. sinensis completely prevented the biofilm formation of S. aureus, but at 16 mg/ml, the aqueous extract completely inhibited the antibiofilm activity of S. aureus isolates. Examining the virulence gene ClfA, which is in controlling S. aureus biofilm formation, the gene expression results showed the isolates treated with the methanolic extracts’ sub-MIC had lower levels of the ClfA gene than the untreated isolates. Progress, rural women's participation in sports will face more opportunities.
Keywords: Antibacterial,Antibiofilm, ClfA, C. sinensis, Phenolic compounds, S. aureus
