Anti-proliferative, Anti-oxidant and Anti-inflammatory Effects of Topical Rutin on Imiquimod-Induced Psoriasis in Mice
Shan Mohammed Khorsheed, Ahmed R Abu Raghif
Psoriasis is a chronic autoimmune dermatosis characterized by thickened, reddish-brown, and peeling skin lesions. To evaluate the potential Anti-proliferative, Anti-oxidant and Anti-psoriatic effects of Rutin 4% ointment in comparison to clobetasol (0.05%) ointment in imiquimod-induced psoriasis in mice model on the basis of histopathological and observational outcomes, as well as biomarkers is the purpose of this research. Fifty healthy adult Swiss albino mice, weighing 24-30 grams and aged 11 to 15 weeks were randomly divided into five groups (n=10); each group contained ten mice with shaved dorsal skin; apparently healthy mice group (group I) did not receive any medication; induced group (group II) received only topical Imiquimod; vehicle control group (group III) received a topical ointment base only; standard group (group IV) received 0.05% Clobetasol ointment only, received topical Rutin 4% ointment (group V). All treatment groups received topical Imiquimod cream for induction on the shaved back for 6 consecutive days; then received topical treatment for 8 days with induction and scoring for skin inflammation severity (scaling, erythema and thickness) was recorded on daily basis, and the animals were sacrificed on day 14. Psoriasis induction was successful by Imiquimod, and psoriasis like lesions developed on the skin. Topical Rutin treatment groups significantly reduced the inflammatory signs of the psoriatic like lesions and these findings were supported by the histopathological examination. The present study showed a significant (P<0.001) decrease in the psoriasis area and severity index score (PASI) and a significant (P<0.001) reduction in the tumor necrosis factor- alpha (TNF- α), vascular endothelial growth factor (VEGF), Interleukin-17 (IL-17), oxidative stress marker Malondialdehyde (MDA) and marker of cell proliferation (Ki67) levels in skin tissue of the Clobetasol and Rutin groups as compared to the induction group. Additionally, histopathological outcomes show a significant (P<0.001) attenuation in the imiquimod- induced psoriasis in mice. Topical Rutin significantly decreased vascular endothelial growth factor levels. At the same time, Rutin showed a more significant reduction in Interleukin-17 (IL-17) levels (P<0.001).
Keywords: Ki-67, MDA, VEGF, TNF-α, Psoriasis, Rutin
