Immunological Evaluation of TGF-β and Osteopontin of Idiopathic Pulmonary Fibrotic patients in Al-Diwaniyah province
Maisam Hilal Hasan, Alaa Mohamed Hasoon
Background: Idiopathic pulmonary fibrosis (IPF) is a hetero-geneous sickness considered via an perversely stimulated immunity system. Present study aimed to evaluate of TGF-β and Osteopontin of IPF sick in Al-Diwaniyah province. Methods: the existing training stayed a case-control training comprised 70 sick who were diagnosed (Clinically, and X-ray) with IPF and 70 healthy control groups. The samples collected from individuals visited Al-Diwaniya teaching hospital in Al-Diwaniyah city. ELISA was used in this study for quantitative determination of Osteopontin and TGF-β in serum samples of participants and done it according to company instruction (BT-LAB). Results: The ELISA test showed important rise in concentration mean of TGF-β and Osteopontin in the sick (751.3 ± 118 ng/ml and 1930.5 respectively) compared to the control (548.6 ± 217 ng/ml and 906.26 ± 252 pg/ml respectively) (P< 0.05). The concentration of TGF-β increased in females (999.38 ± 120 ng/ml) while concentration mean of osteopontin increased in males (2125.20 ± 882 pg/ml). Moreover, the concentration mean of TGF-β and osteopontin increased in elderly patients in age group 4 (759.44 ± 107 ng/ml and 2256.12 ± 759 pg/ml respectively).We found a higher mean of Osteopontin concentration in smoking patients (3636.93± 884 0g/ml ) compared to non-smokers (1638.31 ± 638 pg/ml), in contrast high serum level of TGF-β determine in non- smokers (568.5 ± 111 ng/ml) compared to smokers (433.74± 127 pg/ml) but without significant association (P>0.05). In conclusion: TGF-β and Osteopontin are clearly associated with IPF and their concentrations may be affected by age and gender. Therefore, these indicators can be relied upon as markers in the analysis of IPF.
Keywords: IPF, TGF-β, Osteopontin, ELISA, Al- Diwaniyah
