Chimera Patterns of Escherichia coli lineage with Multi Stress Hardening Resistance to Antibiotics and Ultraviolet Irradiation
Farah Ibrahim Mustafa AL-Salihi, Ali Hassan Ahmed Al-Shammary
Frequency and distribution patterns of diverse and versatile E. coli lineage with dynamic pluripotent virulency to different potent microbicidal stressors like pasteurization regimes cascaded by well-equipped genetic plasticity to evolved to a chimeras with forbidden prohibited resistance to different antibiotics and tolerance to ultraviolet irradiation cold pasteurization were resident within tap waters chain especially within purification stations covered and protected by electromagnetic clouds barriers of biofilm in Baghdad as an emergent bioterrorism hazard catastrophically threatening lifestyles in this mess. These emergent entities unveiled in this torment could be terminated by lytic bacteriophages later as a new combating pathway for hurtling decontamination cascaded by sterilization efficacy dynamic module. Augmented conservative biofilm construction modified protocols were dependent from verified gold standard tissue culture plates to phenotypic plasmids twisters cascaded via verified Congo red assay. Antibiogram dependent antibiotics susceptibility patterns to different drugs of choices with different generations were assigned by Kirby-Bauer disc diffusion method cascaded by VITEK® 2 SYSTEM displayed MIC and MBC thresholds. An experimental design model was verified for susceptibility patterns to cold pasteurization regime via ultraviolet irradiation decontamination scheme. Problematic forbidden recovery dogmas of E. coli lineage from tap waters chains resident within Al-Karkh and Al-Rusafa sectors cascaded by Human UTI clinical cases in Baghdad. Documentary records data unveiled multi stress patterns of recovered isolates to versatile antibiotics with shifting diversity to tolerate irradiation pasteurization. In conclusions: Contamination of tap waters with multi stress hardening E. coli lineage cascaded with similarity genetic indices and phylogenetic tree ancestral relationships with clinical isolates from Human UTI cases as a forbidden complex sequel.
Keywords: E. coli lineage, Stress adaptation, Antibiotics resistance, Ultraviolet irradiation, Tap waters, UTI
