Evaluation the effect of Cefotaxime on gene expression of Eno in Pseudomonas aeruginosa
Mokhtar Jawad Al-Imam, Alaa Saadi Abbood,
Anwer Jaber Faisal, Mohammed S. Abbas
Pseudomonas aeruginosa is one of the most dangerous wound bacteria, and its resistance is being studied. Virulence is as important as removing the organism. Fifty wound samples from Baghdad hospitals were examined and identified by standard tests and VITEK-2. The genus was identified by detecting the particular gene Eno using the polymerase chain reaction (PCR) technology. Based on the findings of cultural and biochemical tests, only 12 isolates were recognized as Pseudomonas aeruginosa, the most common causal agent. Six isolates exhibiting the greatest antibiotic resistance had their minimum inhibitory concentrations (MICs) for Cefotaxime determined using the appropriate diffusion method. We calculated that the minimum inhibitory concentration (MIC) of cefotaxime was (1/8) µg/ml. All isolates had the Eno gene identified by PCR, demonstrating its significance in identifying the P. aeruginosa species and its innate molecular traits. The Eno gene expression was assessed by quantitative reverse transcriptase PCR. When Cefotaxime was applied to the wound samples, the gene expression fold value decreased as compared to the untreated isolates, according to the study.
Keywords: Cefotaxime, Eno, Pseudomonas aeruginosa
