Eco-Friendly Synthesis of Silver Nanoparticles Using Prosopis farcta Fruit Extract and Evaluate Their Biological Applications
Intisar Albandar, Ohood Aqeed Radhi, Sahar A.A. Malik Al-Saadi,
Mohammed A. Mahdi
Recent research has shown increased interest in silver nanoparticles (AgNPs) research due to their promising applications. Since that most bacterial species became resistant to antibiotics that are available locally, they pose a serious threat to healthcare institutions. Thus, the creation of a substitute tool for the management of multi-resistance bacteria becomes imperative. Hence, this paper describes a fast and environmentally friendly method for synthesizing silver nanoparticles (AgPFNPs) using the aqueous solutions of P. Farcta extract and assessing their antimicrobial efficacy against pathogenic bacteria. The GC-MS measurements were carried out to analyze the Prosopis farcta extract's chemical composition. Different The AgPFNPs' absorption band location was scanned using an ultraviolet-visible (UV-vis) spectrophotometer and the Fourier-transform infrared spectroscopy (FTIR) was used to reveal the biomolecules in the plant extract. The size distribution and images of AgPFNPs were investigated using a Zetasizer and scanning electron microscopy (SEM) measurements, respectively. The antibacterial activity of AgPFNPs was evaluated against two types of pathogenic bacteria. The results revealed that P. farcta fruit has eleven phytochemical components discovered in it. The major component was Ethyl. Alpha.-d-glucopyranoside (81.0146 %), while, Catechol was the lower one (1.0296%). In addition, the changing of the reaction mixture's colour from pale yellow to dark brown was an indication of AgPFNP formation. AgPFNPs recorded an absorbance peak at 436 nm and had a proper size of 42.71 (d. nm) and a Z-average of 102 (d. nm). FTIR examination revealed the types of chemical combinations in the P. farcta extract that play a role in the production and surface capping of AgPFNPs. SEM images demonstrated the structure of dispersed AgNPs on the nanoscale. Moreover, AgPFNPs displayed full hemocompatibility with RBCs and exhibited a distinctive antibacterial activity with inhibition zones of 11.77±0.18 mm and 14.07±0.29 mm against Staphylococcus aureus and Escherichia coli, respectively.
Keywords: Nanotechnology, Nanoparticles, Silver nanoparticles, Prosopis farcta, Antibacterial
