The Spectrum of Bacterial Virulence: Analyzing Protease, DNase and Hemolysin Across Diverse Pathogens
Raghad Z. Suleiman, Mujahid Kh Ali, Huda Saleh Khuder
This study investigated the enzymatic activities of proteases, DNases, and hemolysins across five clinically relevant bacterial strains: Among the various bacterial species in the contaminated foods and drinks the most common are – Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pyogenes, and Bacillus cereus. Protease activity was analyzed using casein solution to evaluate the relative optical density, while DNase activity was determined on DNase agar plates observing the clear zones on the agar media and hemolysin activity was checked on blood agar plates. The contention here is that mean diameter of clear zones was determined for die strains and we prevailed to analyze the differences in die enzymatic activities. Our findings also highlighted differences in the bacterial strains studied with regard to a number of factors including protease, DNase and haemolytic activity: P. aeruginosa displayed the highest protease activity while S. aureus and B. cereus were observed to have high DNase activity while S. pyogenes displayed the highest haemolytic activity. The protease and DNase parameters were further analyzed by means of correlation coefficients, and the results revealed a linear correlation between the two at +0.983; however, the hemolysin activity was not directly related to either protease or DNase. Altogether the current observations offer perspectives regarding the numerous strategies that bacteria have adapted in infecting host tissues and producing damage, as well as the significance of studying enzymatic processes in an effort to develop specific pharmacological treatments.
Keywords: Enzymatic activities, Protease, DNase, Hemolysin Bacterial strains, , E. coli, S. aureus, P. aeruginosa, S. pyogenes, B. cereus, Blood agar plates
