Study of Role Flavonoid Compound Isolated from Camellia Sinensis (Black Tea) Extract against Methotrexate Drug Induced Liver Toxicity in Male Rate
Nareman Adeem Shnaa Aljazy, Ihsan Edan Alsaimary,
Alya Jameel Ali Alsaad
The objective of this study was to determine the antioxidant and protective effects of a flavonoid compound derived from Camellia sinensis (Black tea) extract against methotrexate drug-induced liver damage caused by lipid peroxidation (LPO), malondialdehyde (MDA), and MDA in male rats. This experiment was conducted in the postgraduate lab at the University of Kerbala from August to January 1, 2023. On August 11th, 2023, the Camellia sinensis (Black tea) plant was taken from Karbala, and methanol alcohol was used to extract the flavonoid component. leaves for black tea, Eight groups of rats were present. G1-the control group has only received saline solution (0,85%). 100 mg/kg B.W. of flavonoid and 15 mg/kg of MTX were injected into the G2. 15 mg/kg of MTX and 150 mg/kg B.W. of flavonoid are injected into G3. G4: MTX 500 mg/kg and flavonoid injection 200 mg/kg B.W. The G5 injection contains 15 mg/kg of MTXl and 100 mg/kg B.W. of black tea extract. G6-injection with 150 mg/kg B.W. black tea extract and 15 mg/kg MTX G7: 200 mg/kg B.W. of black tea extract and 15 mg/kg of MTX were provided intravenously; G8: 15 mg/kg of MTX was given intravenously. By measuring the effectiveness of the liver enzymes MDA, LPO, and GPX in the blood serum of male rats treated with flavonoids and Black tea leaf and comparing the result with rats treated with methotrexate (MTX), it was found that the concentration of MDA and LPO was significantly higher in G8 (injection with 15 mg/kg of MTX) than in G1 control. Hence, administering MTX with drinking water causes oxidative stress and an increase in ROS by raising MDA and LPO levels in liver tissue and lowering GPX.
Keywords: Black Tea, Methotrexate, Flavonoid
