Evaluation of the Bioactive Properties of the Combined Ethanolic Extracts of Cordyceps militaris and Suaeda maritima


Urarux Romruen
Eakaphun Bangyeekhun

This research work aims to evaluate the bioactive properties of combined ethanolic extracts of Cordyceps militarifits. Seven different ratios of these extracts were prepared and analyzed for their antioxidant and antidiabetic activities. Extracts no. 6 (20:80, CME: SME) and 7 (pure SME) exhibited the highest 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging activity, with IC50 values of 377.66 ±13.97 and 305.28 ±28.70 µg/mL, respectively. This particular extract, number 7, also had the best ability to reactive with ABTS radicals, with an IC50 value of 172.15 ±15.48 µg/mL in the ABTS detection test. In the FRAP assay, extracts no. 6 and 7 displayed the highest antioxidant activity, with IC50 values of 6.30 ±0.80 and 6.48 ±0.22 µg/mL, respectively. Notably, extract no. 1 (pure CME) had the strongest α-amylase inhibitory effect, with an IC50 value of 281.35 ±31.71 µg/mL. Extract no. 6 had a much stronger α-amylase inhibitory effect than extract no. 7. Furthermore, LC-QTOF-MS/MS analysis identified four compounds with potential biological activities in extract no. 6. These findings highlight the potential health benefits of the combined ethanolic extracts of CME and SME and the presence of bioactive compounds in the extracts.


Keywords:Cordyceps militaris, Suaeda maritima, Bioactive activity, Antioxidants, Antidiabetic

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